Linked from

The 16 pages that link to DNA ligase, each with the reason it gives.

• DNA replicationRelated: It joins adjacent fragments into a continuous lagging strand.

• DNA repairRelated: Many repair pathways leave a nick that ligase must seal to restore strand continuity.

• Recombinant DNARelated: It joins compatible DNA ends to make a continuous recombinant molecule.

• Homologous recombinationRelated: Repair synthesis and recombination resolution can leave nicks that require sealing.

• Phosphodiester bondRelated: It restores phosphodiester continuity between adjacent DNA ends.

• Molecular cloningRelated: It covalently joins insert and vector DNA after their ends are aligned.

• Paul BergRelated: DNA ligase supplied the bond-forming step needed to assemble joined DNA molecules.

• Condensation reactionRelated: It catalyzes bond formation in DNA, coupling ligation to release of a leaving group.

• Site-directed mutagenesisRelated: Ligation can seal nicks in mutant DNA products in some mutagenesis workflows.

• Arthur KornbergRelated: Kornberg’s laboratory also helped identify this enzyme, which completes discontinuous DNA synthesis.

• Stanley CohenRelated: Ligase sealed inserted DNA into plasmids used in Cohen and Boyer’s experiments.

• Har Gobind KhoranaRelated: Khorana’s group used ligase in methods for assembling synthetic DNA sequences.

• Aziz SancarRelated: After damaged DNA is replaced, ligase closes the remaining break in the sugar-phosphate backbone.

• Cloning vectorRelated: It seals the inserted fragment into the vector backbone.

• Tsuneko OkazakiRelated: It seals the nicks between Okazaki fragments after their synthesis.